capB [2017-12-08 10:19:47]

capsular polyglutamate biosynthesis

locus: BSU35900

pI: 5.07

mw: 43.79 kDa

protein length: 393 aa

gene length: 1179 bp

function: capsule synthesis

product: poly-gamma-glutamate synthetase

essential: no

synonyms: ywsC, pgsB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.4|Capsule biosynthesis and degradation]

Gene

Coordinates: 3,699,446 → 3,700,627

Phenotypes of a mutant

• no synthesis of the poly-gamma-glutamate capsule [Pubmed|11751809]

The protein

Catalyzed reaction/ biological activity

• synthesis of poly-gamma-glutamate from L-Glu [Pubmed|11751809]

Expression and Regulation

Operons

• genes: [gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]
  description: [Pubmed|11751809]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|19420703], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|19734658], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]

  additional information

  • expression of the operon is increased in '[protein|search|motA]' or '[protein|search|motB]' mutants due to increased [protein|search|DegU] phosphorylation [PubMed|24296669]

Biological materials

Mutant

• MGNA-B262 (ywsC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1261 NBRP B. subtilis, Japan]
• BKE35900 (Δ[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTCGACATCTCC, downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA
• BKK35900 (Δ[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTCGACATCTCC, downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA

References

Reviews

• 20735481,16689787

Original publications

• 11751809,19420703,19389763,16233197,19734658,20564357,20564574,24296669,21965392,27073802